To Study The Efficacy Of Reciprocating Single File System And Multifile Rotary Systems In Elimination Of Root Canal Bacteria And Endotoxins

  • Nisha Garg
  • Paramjeet Kaur
  • Ajay Chhabra
  • Pranav Nayyar
  • Shabnam Negi
  • Ruhani Bhatia
Keywords: Endotoxin, Waveone, Reciproc, Colony Forming Unit, Limulus Amebocyte Lysate test

Abstract

AIM: To study the efficacy of reciprocating single file system and multifile rotary systems in elimination of root canal bacteria and endotoxins.

MATERIAL AND METHOD: Forty permanent single rooted mandibular premolars with straight canals inoculated with Escherichia Coli suspension for 21 days were selected for the study. Teeth were randomly divided into four groups (n=10) according to instrumentation system: Grp. A–Reciproc (VDW); Grp. B–WaveOne (Dentsply Maillefer); Grp. C–MTwo (VDW); and Grp. D–K3( Sybron Endo); Bacterial and endotoxin samples were collected with a sterile paper point before instrumentation  and after instrumentation. Culture methods estimated the colony-forming units (CFU) and the Limulus Amebocyte Lysate test was used for quantification of endotoxins. Results so obtained were calculated and statistically analysed. 

RESULT: Results at S1 concluded that bacteria and endotoxins were found in all of the investigated root canals. After instrumentation all systems were associated with the significant reduction of the bacterial load and endotoxins respectively: Grp. A– Reciproc (88.25% and 89.10%); Grp. B– WaveOne (83.53% and 80.59%); Grp. C– MTwo(79.41% and 75.84%) and Grp. D– K3 (67% and 74.4%). Statistically no difference was found amongst the instrumentation systems regarding bacteria and endotoxin elimination (P <0.05). 

CONCLUSION: The reciprocating single file, Reciproc and WaveOne were as effective as the multifile rotary systems for the eradication of bacteria and endotoxins from root canals.

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Author Biographies

Nisha Garg

Professor  & Head, Department of Conservative Dentistry and Endodontics

Bhojia Dental College and Hospital, Bhud

Baddi (Himachal Pradesh)

Paramjeet Kaur

Post Graduate Student, Department of Conservative Dentistry and Endodontics

Bhojia Dental College and Hospital, Bhud

Baddi (Himachal Pradesh)

Ajay Chhabra

Professor  & Former Principal, Department of Conservative Dentistry and Endodontics

Bhojia Dental College and Hospital, Bhud

Baddi (Himachal Pradesh)

Pranav Nayyar

Associate Professor, Department of Conservative Dentistry and Endodontics

Bhojia Dental College and Hospital, Bhud

Baddi (Himachal Pradesh)

Ruhani Bhatia

Senior Lecturer, Department of Conservative Dentistry and Endodontics

Bhojia Dental College and Hospital, Bhud

Baddi (Himachal Pradesh)

Published
2019-04-18
How to Cite
Nisha Garg, Paramjeet Kaur, Ajay Chhabra, Pranav Nayyar, Shabnam Negi, & Ruhani Bhatia. (2019). To Study The Efficacy Of Reciprocating Single File System And Multifile Rotary Systems In Elimination Of Root Canal Bacteria And Endotoxins. International Healthcare Research Journal, 3(1), 39-43. https://doi.org/10.26440/IHRJ/0301.04.521073
Section
Original Research(s)